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| The Applied Proteome Research Lab (APRL) at UC Irvine has developed a high throughput cloning and protein microarray chip fabrication approach that is useful for profiling immunoreactivity on a large scale. The lab has made more than 30,000 plasmids derived from 30 infectious microorganisms and printed the encoded proteins on microarrays. We have probed an inventory of more than 9,000 sera from infected, vaccinated and healthy people worldwide and derived immunodominant and serodiagnostic antigens from each agent. The goal of this research activity is to develop a more detailed understanding of how the immune system responds to medically important infectious organisms, and to identify serodiagnostic and subunit vaccine antigens. The results from this work show that in addition to identifying vaccine antigen candidates the protein microarray is a rapid and accurate method for defining immunogenicity of vaccine formulations, for distinguishing vaccine "take", determining the level and longevity of protection, and identifying correlates of protection and surrogate endpoints in animal models and in man. |
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