Effector memory T cell in contact with an antigen presenting cell 3 hours after antigen challenge during DTH
Matheu MP, Beeton C, Garcia A, Chi V, Rangaraju S, Safrina O, Monaghan K, Uemura MI, Li D, Pal S, de la Maza LM, Monuki E, Flügel A, Pennington MW, Parker I, Chandy KG, Cahalan MD.
Department of Physiology and Biophysics, University of California Irvine
Official site for Ed Hardy Designer Hoodies, Womens Blouses, Designer T Shirts, Ed Hardy Tees, Ed Hardy Hats, Ed Hardy Shoes and Ed Hardy Caps, swimwearEd HardyEd Hardy Womens JeansEd Hardy Womens Knits
In this experiment, GFP-labeled ovalbumin-specific CCR7-neg effector memory T cells with elevated Kv1.3 channel expression were adoptively transferred into the peritoneal cavity of healthy Lewis rats. Forty eight hour later, these rats were challenged with antigen (ovalbumin conjugated to Texas red) in one ear and with saline in the other ear. Two-photon imaging was used to visualize the green (GFP-labeled) effector memory T cells that had trafficked to the antigen-challenged ear of the rat 3 hours after antigen challenge. In this video, a green (GFP-labeled) effector memory T cell is seen making a contact with a red antigen presenting cell. The APCs appear red because they have taken up the antigen, ovalbumin-Texas red. An average contact is about 20 minutes.
Full Description
Citation
Abstract
Introduction
Results
Discussions
Material Methods
References
Matheu MP, Beeton C, Garcia A, Chi V, Rangaraju S, Safrina O, Monaghan K, Uemura MI, Li D, Pal S, de la Maza LM, Monuki E, Flügel A, Pennington MW, Parker I, Chandy KG, Cahalan MD. Imaging of effector memory T cells during a delayed-type hypersensitivity reaction and suppression by Kv1.3 channel block. Immunity. 2008 Oct;29(4):602-14.
Brief Description