Effector memory T cells moving in inflamed tissue 24 hours after antigen challenge during delayed type hypersensitivity
Matheu MP, Beeton C, Garcia A, Chi V, Rangaraju S, Safrina O, Monaghan K, Uemura MI, Li D, Pal S, de la Maza LM, Monuki E, Flügel A, Pennington MW, Parker I, Chandy KG, Cahalan MD.
Department of Physiology and Biophysics, University of California Irvine
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In this experiment, GFP-labeled ovalbumin-specific CCR7-neg effector memory T cells with elevated Kv1.3 channel expression were adoptively transferred into the peritoneal cavity of healthy Lewis rats. Forty eight hour later, these rats were challenged with antigen (ovalbumin conjugated to Texas red) in one ear and with saline in the other ear. Two-photon imaging was used to visualize the green (GFP-labeled) effector memory T cells that had trafficked to the antigen-challenged ear of the rat 24 hours after antigen challenge. In this video, green effector memory T cells seen moving on the collagen matrix (blue). The antigen presenting cells appear red because they have taken up the antigen, ovalbumin-Texas red.
Matheu MP, Beeton C, Garcia A, Chi V, Rangaraju S, Safrina O, Monaghan K, Uemura MI, Li D, Pal S, de la Maza LM, Monuki E, Flügel A, Pennington MW, Parker I, Chandy KG, Cahalan MD. Imaging of effector memory T cells during a delayed-type hypersensitivity reaction and suppression by Kv1.3 channel block. Immunity. 2008 Oct;29(4):602-14.