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 Brief Description |
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| This video shows a T cell interacting with an antigen presenting cell (APC). In this experiment, MHC class II–restricted B cell (2PK3) hybridomas were pulsed with the antigen (10 ug/ml hen egg lysozyme [HEL]) for 3-12 h. Murine HEL-restricted CD41 T cells (1E5) were cultured for 4-12 h before being loaded for 1 hr at 25oC with 3 uM fura-PE3/AM. The HEL-pulsed 2PK3 B cells were settled onto coverglass with fura-loaded 1E5 T cells. The changes in intracellular calcium concentrations during antigen presentation were imaged on a Zeiss Axiovert 35 microscope equipped with a heated stage maintained at 35oC and a custom-designed beam-splitter. |
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References |
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| Negulescu PA, Krasieva TB, Khan A, Kerschbaum HH, Cahalan MD. Polarity of T cell shape, motility, and sensitivity to antigen. Immunity. 1996 May;4(5):421-30.
Penna A, Demuro A, Yeromin AV, Zhang SL, Safrina O, Parker I, Cahalan MD. The CRAC channel consists of a tetramer formed by Stim-induced dimerization of Orai dimers. Nature. 2008 Nov 6;456(7218):116-20.
Zhang SL, Yu Y, Roos J, Kozak JA, Deerinck TJ, Ellisman MH, Stauderman KA, Cahalan MD. STIM1 is a Ca2+ sensor that activates CRAC channels and migrates from the Ca2+ store to the plasma membrane. Nature. 2005 Oct 6;437(7060):902-5.
Yeromin AV, Zhang SL, Jiang W, Yu Y, Safrina O, Cahalan MD. Molecular identification of the CRAC channel by altered ion selectivity in a mutant of Orai. Nature. 2006 Sep 14;443(7108):226-9.
Cahalan MD, Wulff H, Chandy KG. Molecular properties and physiological roles of ion channels in the immune system. J Clin Immunol. 2001 Jul;21(4):235-52.
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| Feb 10, 2009 |
2015 |
VA:02:17:300:2009 |
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