Effector memory T cells paralyzed in inflamed tissue by ShK-186 treatment: visualization by two-photon imaging
Matheu MP, Beeton C, Garcia A, Chi V, Rangaraju S, Safrina O, Monaghan K, Uemura MI, Li D, Pal S, de la Maza LM, Monuki E, Flügel A, Pennington MW, Parker I, Chandy KG, Cahalan MD.
Department of Physiology and Biophysics, UC Irvine, Irvine, CA 92697
In that case its mcse 2008 time to be alittle cold. Yes they are mcts testking your friends but its getting to the pmp dumps point where they need to get out.
In this experiment, GFP-labeled ovalbumin-specific CCR7-neg effector memory T cells with elevated Kv1.3 channel expression were adoptively transferred into the peritoneal cavity of healthy Lewis rats. Forty eight hour later, these rats were challenged with antigen (ovalbumin conjugated to Texas red) in one ear and with saline in the other ear. Soon after, ShK-186 was administered by subcutaneous injection at 100 ug/kg. A second injection was administered 24 hours later. Two-photon imaging was used to visualize the green T cells that had trafficked to the antigen-challenged ear of the rat. Effector memory T cells did traffic to the ear, but once within the tissue, the cells were immobilized due to blockade of the Kv1.3 channel. In this video, green effector memory T cells are stuck on the collagen matrix (blue) and are unable to interact with antigen presenting cells (red because they have taken up ovalbumin-Texas red).
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Matheu MP, Beeton C, Garcia A, Chi V, Rangaraju S, Safrina O, Monaghan K, Uemura MI, Li D, Pal S, de la Maza LM, Monuki E, Flügel A, Pennington MW, Parker I, Chandy KG, Cahalan MD. Imaging of effector memory T cells during a delayed-type hypersensitivity reaction and suppression by Kv1.3 channel block. Immunity. 2008 Oct;29(4):602-14.
Matheu MP, Beeton C, Parker I, Chandy KG, D Cahalan M. Imaging effector memory T cells in the ear after induction of adoptive DTH. J Vis Exp. 2008 Aug 14;(18). pii: 907. doi: 10.3791/907.
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